Acid fast staining steps

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Acid fast staining – Principle, Procedure, Steps, Results

Acid fast staining Procedure. Within the slide’s border, cover the smears with a piece of paper towel. Place the slide over a heating beaker of water. Make sure the beaker doesn’t boil dry. Allow the slide to steam for 3-5 minutes after soaking the paper towel in carbolfuchsin. Remove the stained paper towel gently. 

Acid-Fast Stain- Principle, Procedure, Interpretation and

May 08, 2015 · Acid-Fast Stain- Principle, Procedure, Interpretation and Examples. It is the differential staining techniques which was first developed by Ziehl and later on modified by Neelsen. So this method is also called Ziehl-Neelsen staining techniques. Neelsen in 1883 used Ziehl’s carbol-fuchsin and heat then decolorized with an acid alcohol, and 

Acid Fast Stain- Principle, Reagents, Procedure and Result

Jun 03, 2021 · Procedure of Acid Fast Stain. Prepare and fix the specimen smear prior to staining. Place a small strip of blotting or filter paper over the top of the specimen, and place the slide over a boiling hot water bath on a mesh surface. Cover the filter paper with the primary stain, carbolfuchsin. Leave the slide on the water bath for 3 to 5 minutes. 


Dec 03, 2017 · PROCEDURE OF ACID FAST STAINING. 1.) Prepare a thin smear of the specimen on a clean grease free glass slide. 2.) Fix the smear onto the slide by passing over the flame of Spirit lamp or Bunsen burner. 3.) Gently flood the smear with Carbol Fuchsin dye for 5 minutes. 4.) Gently heats the slide till fumes appear & keep the smear moisten with dye. 

Ziehl-Neelsen stain | Acid fast stain | Principle | Steps

The method of choice for sputum smear microscopy is Ziehl-Neelsen (Acid fast) stain because it is the only stain that consistently provides good results without requiring special equipment. Cold staining techniques such as the Kinyoun or Tan Thiam Hok methods are not recommended: it has been proven that they hardly allow the detection of acid-fast bacilli (AFB) in paucibacillary … 

How to Acid Stain Concrete (with Pictures) - wikiHow

Oct 21, 2021 · Applying acid stain to concrete can give new life to plain, and otherwise dull looking surfaces. Acid stains can give concrete a look of deep marbling, along with a color unlike any other type of available flooring. Acid staining your concrete surface can be a do-it-yourself weekend project, or you can have professionals come in and do the job. 

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